Think of your own Essay Example | Topics and Well Written Essays - 1000 words

Think of your own - Essay Example E-coins have significantly simplified the activities involved in the exchange of goods and services. There is a growth in the popularity of the e-coin usage. The growth of e-coin users presents Goldman Sachs with a viable opportunity that needs to be utilized (Meiklejohn et al. pp 1-10). For Goldman Sachs to acquire a strategic position in the future investment industry, it is crucial to introduce e-coin services to its portfolio. E-coins have numerous advantages that have contributed to their growing popularity (Meiklejohn et al, pp.20-25). The number of e-coin users has grown at a high rate in recent times. By April 2015, the number of e-coin users stood at over 3million. The number of e-coin transactions is also on the increase with over 120,000 transactions per day as at March 2015. The e-coin market value is also inclining. These trends provide Goldman Sachs an opportunity to utilize in its quest to maintain competitive advantage over its rivals. With such high rates of growth in various e-coin components, the investment industry is expected to react. Investment executives from different firms are examining the e-coin market to assess the feasibility of earning good returns from it. It will be appropriate for Goldman Sachs to act before its competitors since there is growing attention towards this opportunity from various investment companies. E-coin transactions are quick. A typical e-coin transaction only takes 5 minutes. Bank transactions across different borders take up to 5 days. E–coin transactions do not have charge transaction fees. The short time that e-coin transactions take will Ensure that Goldman Sachs handle more transactions that will in turn translate to increased revenues. Transaction costs will also decline since e-coin transactions do not charge transaction fees. The amount of cost minimized will contribute to increasing profits for Goldman Sachs. E-coins are not controlled or regulated by central

Capital Punishment Essay -- essays research papers fc

Getting Rid of the Death Penalty Capital punishment was a corrective measure that was widely used all over the world. It is difficult to pin point the exact date of it's origin but it is a fact that it was practiced intensely during medieval times. Crimes have occurred probably since the beginning of time and therefore there was a need for a counter attack to minimize if not eradicate it completely. Laws were created for this purpose, but like everything man creates they have proved imperfect and in some cases entirely unacceptable. In Britain, as James B Christoph[1] points out, the major cause for this trend was the social and economic chaos brought about by the Industrial Revolution. The spread of poverty as well as child labor, prostitution and other social evils went hand in hand with unexpected wealth, which sparked off crime. The amazing existence of both these extremes caused insecurity among the lawmakers in Britain resulting in the creation of strict rules, making any offense from stealing turnips to cutting down a tree punishable by death. Some examples he sites show just how obscure the law was. In 1801, a 13-year-old boy was publicly hanged for breaking into a house and stealing a spoon. In 1808, a 7-year-old girl was hang at Lynn for setting a house on fire. To express concern over the above examples, surely these minors did not deserve the kind of punishment they received. Were these crimes so heinous and diabolically evil that only through execution would everyone else understand the gravity of contemplating such an act? Passage of time is usually synonymous with change and as usual man is looking for more innovative ways to carry out his daily activities. This pursuit for perfection did not exclude the death penalty. Other methods of carrying out this punishment emerged, some, more blood curdling than the next but still deemed fit by our ancestral "law makers. " Beheading was a common practice for the Romans and the Greeks. They considered it honorable and therefore preferred it to hanging. The instruments used were the axe and the sword. Later on, the English also employed beheading as sited by John Lawrence.1[2] He states that William the Conqueror introduced beheading to England and the first unfortunate person to experience it was a man named Waltheof, Earl of Northumberland, in 1076. Differing from the Rom... ...ifferent countries aborting the act as well as the emergence of more and more organizations against this form of punishment. Recent countries that have abolished capital punishment6 include Azerbaijan, Bulgaria, Canada, Estonia, Lithuania and The United Kingdom all in 1998. The various organizations currently involved include Amnesty International, Equal Justice USA, Canadian Coalition Against the Death Penalty and Catholics Against Capital Punishment. WORKS CITED 1. Christoph, James, page 14, "Capital Punishment and British Politics," The University of Chicago Press, 1962. 2. Lawrence, John, page 28, "A History of Capital Punishment," The Citadel Press, New York, 1960. 3. Lawrence, John, page 69," A History of Capital Punishment," The Citadel Press, New York, 1960. 4. Harpers Magazine, page 29"China's Crimes and Punishment," November 1997. 5. Bradgon, David. "Bring Back Capital Punishment Page," http://www.geocities.com/capitolHill/Lobby/5258/index.html 6. Amnesty International. "List of Abolitionist and Retentionis Countries," http://www.amnesty.org/ailib/intcam/dp/abrelist.htm Capital Punishment Essay -- essays research papers fc Getting Rid of the Death Penalty Capital punishment was a corrective measure that was widely used all over the world. It is difficult to pin point the exact date of it's origin but it is a fact that it was practiced intensely during medieval times. Crimes have occurred probably since the beginning of time and therefore there was a need for a counter attack to minimize if not eradicate it completely. Laws were created for this purpose, but like everything man creates they have proved imperfect and in some cases entirely unacceptable. In Britain, as James B Christoph[1] points out, the major cause for this trend was the social and economic chaos brought about by the Industrial Revolution. The spread of poverty as well as child labor, prostitution and other social evils went hand in hand with unexpected wealth, which sparked off crime. The amazing existence of both these extremes caused insecurity among the lawmakers in Britain resulting in the creation of strict rules, making any offense from stealing turnips to cutting down a tree punishable by death. Some examples he sites show just how obscure the law was. In 1801, a 13-year-old boy was publicly hanged for breaking into a house and stealing a spoon. In 1808, a 7-year-old girl was hang at Lynn for setting a house on fire. To express concern over the above examples, surely these minors did not deserve the kind of punishment they received. Were these crimes so heinous and diabolically evil that only through execution would everyone else understand the gravity of contemplating such an act? Passage of time is usually synonymous with change and as usual man is looking for more innovative ways to carry out his daily activities. This pursuit for perfection did not exclude the death penalty. Other methods of carrying out this punishment emerged, some, more blood curdling than the next but still deemed fit by our ancestral "law makers. " Beheading was a common practice for the Romans and the Greeks. They considered it honorable and therefore preferred it to hanging. The instruments used were the axe and the sword. Later on, the English also employed beheading as sited by John Lawrence.1[2] He states that William the Conqueror introduced beheading to England and the first unfortunate person to experience it was a man named Waltheof, Earl of Northumberland, in 1076. Differing from the Rom... ...ifferent countries aborting the act as well as the emergence of more and more organizations against this form of punishment. Recent countries that have abolished capital punishment6 include Azerbaijan, Bulgaria, Canada, Estonia, Lithuania and The United Kingdom all in 1998. The various organizations currently involved include Amnesty International, Equal Justice USA, Canadian Coalition Against the Death Penalty and Catholics Against Capital Punishment. WORKS CITED 1. Christoph, James, page 14, "Capital Punishment and British Politics," The University of Chicago Press, 1962. 2. Lawrence, John, page 28, "A History of Capital Punishment," The Citadel Press, New York, 1960. 3. Lawrence, John, page 69," A History of Capital Punishment," The Citadel Press, New York, 1960. 4. Harpers Magazine, page 29"China's Crimes and Punishment," November 1997. 5. Bradgon, David. "Bring Back Capital Punishment Page," http://www.geocities.com/capitolHill/Lobby/5258/index.html 6. Amnesty International. "List of Abolitionist and Retentionis Countries," http://www.amnesty.org/ailib/intcam/dp/abrelist.htm

Restriction Endonuclease Digestion of Plasmid Dna

Introduction: With the execution of this experiment, we began to go deeper into the Cell and Molecular Biology course. The main focus of the experiment would be how the Restriction Endonucleases cleave the strands of DNA. For this experiment, pBR322 was the specimen to use. Restriction Endonucleases work by cleaving the sugar phosphate backbone of specific DNA sites. Restriction enzymes that have been isolated from bacteria have a defensive role. This idea is illustrated when an attacking foreign cell DNA is trying to alter the bacteria; restriction enzymes cleave the DNA rendering it inert.The second part of the experiment deals with Gel Electrophoresis. The samples are loaded into wells on an 1% agarose slab and subjected to electrical currents both positive and negative. Our current target here is DNA, therefore since nucleic acid as a negative charge, the bands will migrate toward the positive cathode. This process of migration is called sieving and smaller strands move faster th an longer strands due to their ease in going through the gel. The objectives of the experiment include:Learning the principles behind Restriction Enzymes and Gel Electrophoresis Applying the concepts in the experiment to produce bands at the end of the Gel Electrophoresis stage Interpreting what these bands mean with accordance to how the plasmid was cleaved Methods and Materials: For the experiment we used several restriction endonucleases (BamHI, EcoRI, HindIII, PstI, ScaI, SaII), ppBR322 plasmid DNA, TAE/TE Buffer, DNA Ladder (50 Bp), Restriction Buffers, 1g of Agarose, 700ml of Distilled H2O. Equipment used for the experiment included: Agarose Gel Electrophoresis System, Uv-vis illuminator and Camera or a Gel doc-it documentation system.The first procedure began by adding 8. 5  µL sterile distilled H2O, 1. 0 µL of the appropriate 10x buffer, 1. 0 µL combination of the restriction endonucleases and 1. 0 µL of pBR322 plasmid DNA (the DNA would be added last) in 5 separate 1. 5ml microcentrifuge tubes, one tube is not to have an RE in it. The mixture was then incubated for 1 hour at 37  °C. No dry block heater was available so body heat was used. After incubation, 2 µL of gel loading dye (Bromphenol Blue) was added to each mixture and loaded in 1% agarose gel. The 50bp DNA ladder was placed in lane 1.It was then subjected to electrophoresis at 100V 250mA 50W. Agarose gel was prepared by dissolving 1g of agarose gel powder in 100mL distilled H2O in a microwave over. It was then cooled at 60 °C then poured in a gel casting tray. A comb was then put and the gel was left to solidify. Afterwards, the gel casting tray was placed into the submarine gel electrophoresis system. The TAE buffer was then placed. The samples were then loaded from left to right starting with the DNA ladder on lane with and the sample without any restriction enzyme on the extreme right.It was then covered and the anodes were connected on the side of the walls. They were connec ted to the power supply set at 100V 250 mA 50W and then run. When the tracking dye reached near the end point, the power supply was turned off. The gel was then removed and transferred into a developing try containing a 10 µL ethidium bromide pero 100ml buffer. It was then shook for 15 minutes. The get was then transferred to the documentation system and Rf values were measured. Pictures were taken and the gel was immersed in hypocholorite (chlorox) solution before discarding. Results and DiscussionThe group did not include a mix without restriction enzymes because doing so will lead to undigested or incompletely digested DNA. The DNA methyltransferase (DNA MTase) family of enzymes catalyze the transfer of a methyl group to DNA. DNA methylation serves a wide variety of biological functions. All the known DNA methyltransferases use S-adenosyl methionine (SAM) as the methyl donor. In prokaryotes, the major role of DNA methylation is to protect host DNA against degradation by restric tion enzymes. In eukaryotes, DNA methylation has been implicated in the control of several cellular processes, including ifferentiation, gene regulation, and embryonic development. Structural work on HhaI DNA methyltransferase demonstrates that the substrate nucleotide is completely flipped out of the helix during the modification reaction and has provided much insight into the enzymatic properties of S-adenosyl-L-methionine (SAM)-dependent DNA-modifying enzymes. Structural comparison of three enzymes, HhaI C5-cytosine methyltransferase, TaqI N6-adenine methyltransferase, and catechol O-methyltransferase, reveals a striking similarity in protein folding and indicates that many SAM-dependent methyltransferases have a common catalytic-domain structure.This feature permits the prediction of tertiary structure for other DNA, RNA, protein, and small-molecule methyltransferases from their amino acid sequences, including the eukaryotic CpG methyltransferases. Ethidium bromide is an interca lating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. It is commonly abbreviated as â€Å"EtBr†, which is also an abbreviation for bromoethane.When exposed to ultraviolet light, it will fluoresce with an orange colour, intensifying almost 20-fold after binding to DNA. Ethidium bromide is an â€Å"intercalating dye†, that is, it is able to slip itself into the DNA while essentially stacking itself between the bases of the helix. When it is inserted into the DNA, it becomes much more fluorescent when exposed to ultraviolet light as compared to ethidium bromide just in solution. So we can use it to visualize the DNA that has been resolved on a gel by electrophoresis.

Similarities And Differences Between Starbucks And Walmart

â€Å"Every business has its own business strategy which leads the company towards success and accomplishment.† (Stankeviciute et al., 2012, p. 1200). The following analysis will examine the similarities and differences between Starbucks and Walmart in regards to people, profit, and planet. People Legal Compliance. Determining the relationship between a company and people can be evaluated in several ways. Legal compliance issues not only provide powerful insight into an organization’s ethical business practices, but also how these engagements affect people within the company. In Chou vs. Starbucks, it was determined that Starbucks misclassified shift supervisors as nonexempt customer-service employees in violation of California law enabling supervising employees to share in tip pools with non-management baristas. (Brody et al., 2008). Here, a significant amount of employees were prevented from receiving fair compensation in this tip-sharing model. Many employees are reliant upon tips as part of their compensation and allowing managers with higher pay rates to also share tips diminishes the ability of baristas to earn a proper wage. Starbucks has also struggled to comply with tax codes as seen through its failure to pay the appropriate amount of taxes owed based on profi ts over a fourteen year period. (Brody et al., 2008; Shaheen, 2012). Not only did Starbucks consciously engaged in tax avoidance, but also did so consistently for over a decade. Tax dollars are used to promote theShow MoreRelatedEssay on Csr: Sustainability Practices- Walmart vs. Starbucks1815 Words   |  8 Pagesand the Triple Bottom Line of the corporations Wal-Mart and Starbucks. By analyzing the key differences not only in their values, but the application of their stated values, they can then be judged as to the superiority of their systemic approaches to sustainability. 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